Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.

Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.
The efficacy of various vaccination schedules was evaluated in 17-day-old Pekin geese utilizing an experimental inactivated entire virus vaccine based mostly on the H5N9 A/hen/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing an artificial HA gene from an Asian H5N1 isolate (A/hen/Indonesia/7/2003).
Full safety towards scientific indicators and shedding was induced by the totally different vaccination schemes. Nonetheless, the broadest antibody response and the bottom antibody improve after problem have been noticed within the group of geese whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, suggesting that this prime-boost technique induced optimum immunity towards H5N1 and minimal viral replication after problem in geese. As well as, this prime-boost vaccination scheme was proven to be immunogenic in 1-day-old ducklings.

Danger elements and traits of H5N1 Extremely Pathogenic Avian Influenza (HPAI) post-vaccination outbreaks.

Extremely pathogenic avian influenza (HPAI) virus H5N1 is now endemic in South-East Asia however HPAI management strategies differ between international locations. A widespread HPAI vaccination marketing campaign that began on the finish of 2005 in Viet Nam resulted within the cessation of poultry and human circumstances, however in 2006/2007 extreme HPAI outbreaks re-emerged.
On this research we investigated the sample of this primary post-vaccination epidemic in southern Viet Nam figuring out a spatio-temporal cluster of outbreak prevalence and estimating spatially smoothed incidence charges of HPAI. Spatial threat elements related to HPAprevalence have been recognized.
Medium-level poultry density resulted in an elevated outbreak threat (Odds ratio (OR) = 5.4, 95% confidence interval (CI): 1.6-18.9) but in addition climate-vegetation elements performed an necessary position: medium-level normalised distinction vegetation indices throughout the wet season from Could to October have been related to greater threat of HPAI outbreaks (OR = 3.7, 95% CI: 1.7-8.1), in all probability as a result of temporal flooding might need offered appropriate circumstances for the re-emergence of HPAI by increasing the virus distribution within the surroundings and by enlarging areas of potential contacts between home waterfowl and wild birds.
However, a number of agricultural manufacturing elements, comparable to candy potatoes yield, elevated buffalo density, in addition to elevated electrical energy provide have been related to decreased threat of HPAI outbreaks. This illustrates that preventive management measures for HPAI ought to embrace a promotion of low-risk agricultural administration practices in addition to enchancment of the infrastructure in village households. Improved HPAI vaccination efforts and protection ought to give attention to medium poultry density areas and on the pre-monsoon time interval.

Investigation of the organic indicator for vaccine efficacy towards extremely pathogenic avian influenza (HPAI) H5N1 virus problem in mice and ferrets.

To research the organic indicator for vaccine efficacy towards HPAI H5N1 virus problem of various clades, two inactivated whole-virus H5N1 vaccines containing the hemagglutinin (HA) and neuraminidase (NA) genes of both clade 2.2 A/EM/Korea/W149/06 (RgKoreaW149/06 x PR8) or clade 2.5 A/Ck/Korea/ES/03 (RgKoreaES223N/03XPR8) virus within the background of A/PR/8/34 (H1N1) have been generated by reverse genetics.
 Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.
Administration of the vaccines (2-dose 1.77, 3.5, 7.5 or 15microg of HA) elicited excessive HI titers in a dose-dependent method. Mice immunized with RgKoreaW149/06 x PR8 have been fully shielded from problem towards wild-type A/EM/Korea/W149/06 with out scientific indicators of an infection. RgKoreaES223N/03XPR8 couldn’t shield mice at 1.77microg whereas all immunized ferrets have been fully protected. Two-dose (7.5microg) vaccinated mice (HI titer>> or =320) and triple dose (7.5 microg) vaccinated ferrets with RgKoreaES223N/03xPR8 (HI titer>> or =640) protected vaccine recipients from mortality, inhibited nasal virus shedding and restricted influenza virus tropism.
Thus, these vaccines offered cross-protectivity in each fashions. Extra importantly, these outcomes collectively advised a constructive correlation between vaccine-induced HI titers and inhibition of virus shedding together with block of viral proliferation in main organs towards a heterologous HPAI H5N1 virus. Though creating applied sciences or strategies that can allow the discount of administration dose/frequency stays to be resolved, our research demonstrated a substantial organic marker >> or =640 HI titer) for full safety of the vaccinated hosts that would present a preliminary foundation for the evaluation of full immunization.

Mucosal immunity induced by adenovirus-based H5N1 HPAI vaccine confers safety towards a deadly H5N2 avian influenza virus problem.

Improvement of efficient vaccines towards extremely pathogenic avian influenza (HPAI) H5N1 viruses is a worldwide public well being precedence. Contemplating the problem in predicting HPAI H5N1 pandemic strains, one technique used of their design consists of the event of formulations with the capability of eliciting broad cross-protective immunity towards a number of viral antigens.
To this finish we constructed a replication-defective recombinant adenovirus-based avian influenza virus vaccine (rAdv-AI) expressing the codon-optimized M2eX-HA-hCD40L and the M1-M2 fusion genes from HPAI H5N1 human isolate. Though there have been no vital variations within the systemic immune responses noticed between the intramuscular prime-intramuscular increase routine (IM/IM) and the intranasal prime-intramuscular increase routine (IN/IM), IN/IM induced stronger CD8(+) T cell and antibody responses at mucosal websites than the IM/IM vaccination, leading to more practical safety towards deadly H5N2 avian influenza (AI) virus problem.

FITC antibody (IgG fraction)

20C-CR1056SP 1 ml
EUR 241
Description: Rabbit polyclonal FITC antibody

Rabbit Anti-Swine/Porcine Parvovirus (PPV) VP2 IgG, aff pure (IgG fraction)

PPVVP21-A 100 ug
EUR 482

Goat anti-rabbit IgG-HRP

E22NR02-1 100ul
EUR 343

Rabbit Ig fraction

31R-1018 10 mg
EUR 114
Description: Rabbit Ig fraction control or blocking reagent

Rabbit Ig fraction

31R-1042 10 mg
EUR 114
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent

Sheep Anti-Human Plasminogen Activator Inhibitor 1 (PAI-1) IgG, pure

PAI21-A 100 ul
EUR 408

Monoclonal Anti-Human Plasminogen Activator Inhibitor 1 (PAI-1) IgG, pure

PAI22-M 100 ul
EUR 482

Human PAI-1/tPA AssayMax ELISA Kit

EP1105-1 96 Well Plate
EUR 417

Pyruvate Kinase antibody (IgG fraction)

20C-CR7017GP 1 mL
EUR 235
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Pyruvate Kinase antibody (IgG fraction)

20R-PG010 1 ml
EUR 235
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

anti- PAI-1 antibody

FNab06114 100µg
EUR 548.75
  • Recommended dilution: WB: 1:500 - 1:2000
  • IHC: 1:50 - 1:500
  • Immunogen: serpin peptidase inhibitor, clade E(nexin, plasminogen activator inhibitor type 1), member 1
  • Uniprot ID: P05121
  • Gene ID: 5054
  • Research Area: Cancer, Cardiovascular, Metabolism
Description: Antibody raised against PAI-1

Anti-PAI-1 antibody

PAab06114 100 ug
EUR 386

Anti-PAI-1 antibody

STJ16100781 100 µg
EUR 604

Anti-PAI-1 antibody

STJ94934 200 µl
EUR 197
Description: Rabbit polyclonal to PAI-1.

Ibrutinib drug-Human IgG Conjugate

IBT17-IgG 100 ug
EUR 529

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Anti-Human IgG

DB-173-1 1 ml
EUR 613
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-1 1 ml
EUR 613
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)

E0L3012-1 500ul
EUR 128

Rabbit anti-goat IgG(H+L)-HRP

E22NG02-1 100ul
EUR 343

unconjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1041-1 1mg
EUR 101

Plasminogen Activator Inhibitor 1 (PAI-1), hexapeptide, Human

SP-55196-1 0.5 mg
EUR 141

Rabbit PAI 1 ELISA Kit

ERTP0035 96Tests
EUR 521

Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit

EP1100-1 96 Well Plate
EUR 417

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins (HCPs) IgG (Low molecular weight fraction, soluble fraction affinity pure)

730-145-11A 100 ug
EUR 457

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins IgG (HCPs) (High molecular weight fraction, soluble fraction, affinity pure)

730-145-12A 100 ug
EUR 457

Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone#RM104

M04575-1 100ug
EUR 375
Description: Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone#RM104 tested in WB (nonreduced), IP, ICC, IHC, FC, ELISA , reactive to Mouse

CF633 Rabbit anti-human IgG(H+L), highly cross-adsorbed

20066-1 50uL
EUR 125
Description: Minimum order quantity: 1 unit of 50uL

Biotin Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1020-1 1ml
EUR 131

HRP Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1070-1 1ml
EUR 233

TRITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1092-1 1ml
EUR 334

FITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1114-1 1mL
EUR 202

Rabbit anti Human IgG

40C-CB0943 1 mg
EUR 249
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0950 5 mg
EUR 381
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0971 2 mg
EUR 322
Description: Rabbit anti Human IgG secondary antibody

Rabbit Anti Human IgG

E61I00101 100ug
EUR 343

Rabbit Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Rb-48T 48T
EUR 337
  • Should the Rabbit Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Rb-96T 96T
EUR 426
  • Should the Rabbit Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Rb-48Tests 48 Tests
EUR 331

Rabbit Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Rb-96Tests 96 Tests
EUR 451

CF555 goat anti-rabbit IgG(H+L), 2mg/mL

20033-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF647 goat anti-rabbit IgG(H+L), 2mg/mL

20043-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

unconjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1039-1 1mg
EUR 101

unconjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1040-1 1mg
EUR 101

unconjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1042-1 1mg
EUR 101

unconjugated Rabbit Anti-mouse IgG (H+L) secondary antibody

BA1048-1 1mg
EUR 101

HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit

SV0002-1 10ml/kit
EUR 202

Human Recombinant PAI-1

6377-100
EUR 403

Recombinant Human PAI-1

P0142 100ug
EUR 522.36
  • Formulation: pH7.4, Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose
  • Reconstitution: Sterile distilled water
  • Purity: Greater than 95% by SDS-PAGE gel analyses
  • Uniprot ID: P05121
Description: Recombinant Human protein for PAI-1

Rabbit PAI ELISA Kit

ERTP0600 96Tests
EUR 521

Rabbit Anti-Human IgG (Fc) IgG, unlabeled

10119-R 0.5 mg
EUR 141

CF647 rabbit anti-goat IgG(H+L), 2 mg/mL

20049-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF660C goat anti-rabbit IgG(H+L), 2 mg/mL

20053-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF660R goat anti-rabbit IgG(H+L), 2 mg/mL

20055-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF680 rabbit anti-goat IgG(H+L), 2 mg/mL

20068-1 50uL
EUR 132
Description: Minimum order quantity: 1 unit of 50uL

CF750 goat anti-rabbit IgG(H+L), 2 mg/mL

20073-1 50uL
EUR 132
Description: Minimum order quantity: 1 unit of 50uL

CF405S goat anti-rabbit IgG(H+L), 2 mg/mL

20082-1 50ul
EUR 120
Description: Minimum order quantity: 1 unit of 50ul

CF640R rabbit anti-goat IgG(H+L), 2 mg/mL

20090-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF568 goat anti-rabbit IgG(H+L), 2 mg/mL

20102-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF568 rabbit anti-goat IgG(H+L), 2 mg/mL

20107-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF594 goat anti-rabbit IgG(H+L), 2 mg/mL

20112-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF633 goat anti-rabbit IgG(H+L), 2 mg/mL

20122-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF633 rabbit anti-goat IgG(H+L), 2 mg/mL

20128-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF350 goat anti-rabbit IgG(H+L), 2 mg/mL

20141-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF405M goat anti-rabbit IgG(H+L), 2 mg/mL

20181-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

Biotin goat anti-rabbit IgG(H+L), 2 mg/mL

20185-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF640R goat anti-rabbit IgG(H+L), 2 mg/mL

20202-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF488A chicken anti-rabbit IgG(H+L), 2 mg/mL

20209-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF594 chicken anti-rabbit IgG(H+L), 2 mg/mL

20223-1 50uL
EUR 125
Description: Minimum order quantity: 1 unit of 50uL

CF633 chicken anti-rabbit IgG(H+L), 2 mg/mL

20224-1 50uL
EUR 125
Description: Minimum order quantity: 1 unit of 50uL

CF543 goat anti-rabbit IgG(H+L), 2 mg/mL

20309-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

CF543 rabbit anti-goat IgG(H+L), 2 mg/mL

20315-1 50uL
EUR 120
Description: Minimum order quantity: 1 unit of 50uL

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1003-1 1ml
EUR 131

Biotin Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1005-1 1ml
EUR 131

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1006-1 1ml
EUR 131

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1054-1 1ml
EUR 233

HRP Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1058-1 1ml
EUR 233

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1060-1 1ml
EUR 233

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1090-1 1ml
EUR 334

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1091-1 1ml
EUR 334

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1105-1 1mL
EUR 202

FITC Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1108-1 1mL
EUR 233

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1110-1 1mL
EUR 202

DyLight®488 Conjugated Goat Anti-rabbit IgG (H+L)

BA1127-1 1ml
EUR 334

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)

BA1142-1 1ml
EUR 334

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody

BM2004-1 1ml
EUR 344

Anti-PAI-2 antibody

STJ94935 200 µl
EUR 197
Description: Rabbit polyclonal to PAI-2.

Anti-PAI-3 antibody

STJ94936 200 µl
EUR 197
Description: Rabbit polyclonal to PAI-3.

Rabbit anti Human IgG (FITC)

43C-CB0944 1 mg
EUR 311
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0945 1 mg
EUR 348
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0947 1 mg
EUR 340
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0948 1 mg
EUR 417
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit anti Human IgG (FITC)

43C-CB0951 2 mg
EUR 273
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0952 2 mg
EUR 278
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0954 2 mg
EUR 340
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0955 2 mg
EUR 361
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit anti Human IgG (FITC)

43C-CB0965 2 mg
EUR 291
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0966 2 mg
EUR 291
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0968 2 mg
EUR 359
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0969 2 mg
EUR 369
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit Anti Human IgG-Biotin

E61I00102 1mg
EUR 499

Rabbit Anti Human IgG-HRP

E61I00103 1mg
EUR 499

Rabbit Anti Human IgG-FITC

E61I00104 1mg
EUR 611

Rabbit Anti-Human IgG FC

C020221-1mg 1mg
EUR 269

Rabbit Anti-Human IgG FC

C020221-50mg 50mg
EUR 6015

PAI 1 antibody

20R-1823 100 ug
EUR 673
Description: Rabbit polyclonal PAI 1 antibody

PAI-1 Antibody

5579-100
EUR 338

PAI 1 Antibody

abx216912-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

PAI 1 Antibody

AF5176 200ul
EUR 304
Description: PAI 1 Antibody detects endogenous levels of total PAI 1.

PAI 1 Antibody

ABF5176 100 ug
EUR 438

Rabbit anti-Saccharomyces Cerevisiae Host Cell Proteins (HCPs) IgG (Low molecular weight fraction, affinity pure)

760-160-11A 100 ug
EUR 457

Rabbit anti-Saccharomyces Cerevisiae Host Cell Proteins (HCPs) IgG (High molecular weight fraction, affinity pure)

760-160-12A 100 ug
EUR 457

Rabbit Anti-HIV-1 gp41 IgG

AB-16010 1 ml
EUR 347

Rabbit IgG Control Sera

20009-1-200 0.5 ml
EUR 103

Rabbit IgG-Agarose conjugate

20009-AS-1 0.5 ml
EUR 164

CF488A Rabbit anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL

20071-1 50uL
EUR 137
Description: Minimum order quantity: 1 unit of 50uL
These findings counsel that the methods used to induce multi-antigen-targeted mucosal immunity, comparable to IN/IM supply of rAdv-AI, could also be a promising strategy for creating broad protecting vaccines that could be more practical towards the brand new HPAI pandemic strains.

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