Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.

Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.
The efficacy of various vaccination schedules was evaluated in 17-day-old Pekin geese utilizing an experimental inactivated entire virus vaccine based mostly on the H5N9 A/hen/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing an artificial HA gene from an Asian H5N1 isolate (A/hen/Indonesia/7/2003).
Full safety towards scientific indicators and shedding was induced by the totally different vaccination schemes. Nonetheless, the broadest antibody response and the bottom antibody improve after problem have been noticed within the group of geese whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, suggesting that this prime-boost technique induced optimum immunity towards H5N1 and minimal viral replication after problem in geese. As well as, this prime-boost vaccination scheme was proven to be immunogenic in 1-day-old ducklings.

Danger elements and traits of H5N1 Extremely Pathogenic Avian Influenza (HPAI) post-vaccination outbreaks.

Extremely pathogenic avian influenza (HPAI) virus H5N1 is now endemic in South-East Asia however HPAI management strategies differ between international locations. A widespread HPAI vaccination marketing campaign that began on the finish of 2005 in Viet Nam resulted within the cessation of poultry and human circumstances, however in 2006/2007 extreme HPAI outbreaks re-emerged.
On this research we investigated the sample of this primary post-vaccination epidemic in southern Viet Nam figuring out a spatio-temporal cluster of outbreak prevalence and estimating spatially smoothed incidence charges of HPAI. Spatial threat elements related to HPAprevalence have been recognized.
Medium-level poultry density resulted in an elevated outbreak threat (Odds ratio (OR) = 5.4, 95% confidence interval (CI): 1.6-18.9) but in addition climate-vegetation elements performed an necessary position: medium-level normalised distinction vegetation indices throughout the wet season from Could to October have been related to greater threat of HPAI outbreaks (OR = 3.7, 95% CI: 1.7-8.1), in all probability as a result of temporal flooding might need offered appropriate circumstances for the re-emergence of HPAI by increasing the virus distribution within the surroundings and by enlarging areas of potential contacts between home waterfowl and wild birds.
However, a number of agricultural manufacturing elements, comparable to candy potatoes yield, elevated buffalo density, in addition to elevated electrical energy provide have been related to decreased threat of HPAI outbreaks. This illustrates that preventive management measures for HPAI ought to embrace a promotion of low-risk agricultural administration practices in addition to enchancment of the infrastructure in village households. Improved HPAI vaccination efforts and protection ought to give attention to medium poultry density areas and on the pre-monsoon time interval.

Investigation of the organic indicator for vaccine efficacy towards extremely pathogenic avian influenza (HPAI) H5N1 virus problem in mice and ferrets.

To research the organic indicator for vaccine efficacy towards HPAI H5N1 virus problem of various clades, two inactivated whole-virus H5N1 vaccines containing the hemagglutinin (HA) and neuraminidase (NA) genes of both clade 2.2 A/EM/Korea/W149/06 (RgKoreaW149/06 x PR8) or clade 2.5 A/Ck/Korea/ES/03 (RgKoreaES223N/03XPR8) virus within the background of A/PR/8/34 (H1N1) have been generated by reverse genetics.
 Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.
Administration of the vaccines (2-dose 1.77, 3.5, 7.5 or 15microg of HA) elicited excessive HI titers in a dose-dependent method. Mice immunized with RgKoreaW149/06 x PR8 have been fully shielded from problem towards wild-type A/EM/Korea/W149/06 with out scientific indicators of an infection. RgKoreaES223N/03XPR8 couldn’t shield mice at 1.77microg whereas all immunized ferrets have been fully protected. Two-dose (7.5microg) vaccinated mice (HI titer>> or =320) and triple dose (7.5 microg) vaccinated ferrets with RgKoreaES223N/03xPR8 (HI titer>> or =640) protected vaccine recipients from mortality, inhibited nasal virus shedding and restricted influenza virus tropism.
Thus, these vaccines offered cross-protectivity in each fashions. Extra importantly, these outcomes collectively advised a constructive correlation between vaccine-induced HI titers and inhibition of virus shedding together with block of viral proliferation in main organs towards a heterologous HPAI H5N1 virus. Though creating applied sciences or strategies that can allow the discount of administration dose/frequency stays to be resolved, our research demonstrated a substantial organic marker >> or =640 HI titer) for full safety of the vaccinated hosts that would present a preliminary foundation for the evaluation of full immunization.

Mucosal immunity induced by adenovirus-based H5N1 HPAI vaccine confers safety towards a deadly H5N2 avian influenza virus problem.

Improvement of efficient vaccines towards extremely pathogenic avian influenza (HPAI) H5N1 viruses is a worldwide public well being precedence. Contemplating the problem in predicting HPAI H5N1 pandemic strains, one technique used of their design consists of the event of formulations with the capability of eliciting broad cross-protective immunity towards a number of viral antigens.
To this finish we constructed a replication-defective recombinant adenovirus-based avian influenza virus vaccine (rAdv-AI) expressing the codon-optimized M2eX-HA-hCD40L and the M1-M2 fusion genes from HPAI H5N1 human isolate. Though there have been no vital variations within the systemic immune responses noticed between the intramuscular prime-intramuscular increase routine (IM/IM) and the intranasal prime-intramuscular increase routine (IN/IM), IN/IM induced stronger CD8(+) T cell and antibody responses at mucosal websites than the IM/IM vaccination, leading to more practical safety towards deadly H5N2 avian influenza (AI) virus problem.

Rabbit Ig fraction

31R-1018 10 mg
EUR 136.8
Description: Rabbit Ig fraction control or blocking reagent

Rabbit Ig fraction

31R-1042 10 mg
EUR 136.8
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Pyruvate Kinase antibody (IgG fraction)

20C-CR7017GP 1 mL
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Pyruvate Kinase antibody (IgG fraction)

20R-PG010 1 ml
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Human PAI-1/tPA AssayMax ELISA Kit

EP1105-1 96 Well Plate
EUR 500.4

Ibrutinib drug-Human IgG Conjugate

IBT17-IgG 100 ug
EUR 634.8

Sheep Anti-Human Plasminogen Activator Inhibitor 1 (PAI-1) IgG, pure

PAI21-A 100 ul
EUR 489.6

Monoclonal Anti-Human Plasminogen Activator Inhibitor 1 (PAI-1) IgG, pure

PAI22-M 100 ul
EUR 578.4

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Anti-PAI-1 antibody

PAab06114 100 ug
EUR 463.2

anti- PAI-1 antibody

FNab06114 100µg
EUR 658.5
Description: Antibody raised against PAI-1

Anti-PAI-1 antibody

STJ94934 200 µl
EUR 236.4
Description: Rabbit polyclonal to PAI-1.

Anti-PAI-1 antibody

STJ16100781 100 µg
EUR 724.8

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)

E0L3012-1 500ul
EUR 153.6

Rabbit anti-goat IgG(H+L)-HRP

E22NG02-1 100ul
EUR 411.6

unconjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1041-1 1mg
EUR 121.2

Anti-Human IgG

DB-173-1 1 ml
EUR 735.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-1 1 ml
EUR 735.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Plasminogen Activator Inhibitor 1 (PAI-1), hexapeptide, Human

SP-55196-1 0.5 mg
EUR 169.2

Rabbit PAI 1 ELISA Kit

ERTP0035 96Tests
EUR 625.2

Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit

EP1100-1 96 Well Plate
EUR 500.4

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins (HCPs) IgG (Low molecular weight fraction, soluble fraction affinity pure)

730-145-11A 100 ug
EUR 548.4

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins IgG (HCPs) (High molecular weight fraction, soluble fraction, affinity pure)

730-145-12A 100 ug
EUR 548.4

Rabbit Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Rb-48T 48T
EUR 404.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Rb-96T 96T
EUR 511.2
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rabbit Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Rb-48Tests 48 Tests
EUR 397.2

Rabbit Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Rb-96Tests 96 Tests
EUR 541.2

CF633 Rabbit anti-human IgG(H+L), highly cross-adsorbed

20066-1 50uL
EUR 150
Description: Minimum order quantity: 1 unit of 50uL

Biotin Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1020-1 1ml
EUR 157.2

HRP Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1070-1 1ml
EUR 279.6

TRITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1092-1 1ml
EUR 400.8

FITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody

BA1114-1 1mL
EUR 242.4

Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone#RM104

M04575-1 100ug
EUR 450
Description: Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone#RM104 tested in WB (nonreduced), IP, ICC, IHC, FC, ELISA , reactive to Mouse

Rabbit anti Human IgG

40C-CB0943 1 mg
EUR 298.8
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0950 5 mg
EUR 457.2
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0971 2 mg
EUR 386.4
Description: Rabbit anti Human IgG secondary antibody

Rabbit Anti Human IgG

E61I00101 100ug
EUR 411.6

CF555 goat anti-rabbit IgG(H+L), 2mg/mL

20033-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF647 goat anti-rabbit IgG(H+L), 2mg/mL

20043-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

unconjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1039-1 1mg
EUR 121.2

unconjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1040-1 1mg
EUR 121.2

unconjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1042-1 1mg
EUR 121.2

unconjugated Rabbit Anti-mouse IgG (H+L) secondary antibody

BA1048-1 1mg
EUR 121.2

HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit

SV0002-1 10ml/kit
EUR 242.4

Rabbit plasminogen activator inhibitor 1, PAI-1 ELISA Kit

1-CSB-E06978Rb
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Rabbit plasminogen activator inhibitor 1, PAI-1 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit PAI ELISA Kit

ERTP0600 96Tests
EUR 625.2

Human Recombinant PAI-1

6377-100
EUR 483.6

Recombinant Human PAI-1

P0142 100ug
EUR 626.83
Description: Recombinant Human protein for PAI-1

Rabbit Anti-Human IgG (Fc) IgG, unlabeled

10119-R 0.5 mg
EUR 169.2

Rabbit IgG Control Sera

20009-1-200 0.5 ml
EUR 123.6

Rabbit IgG-Agarose conjugate

20009-AS-1 0.5 ml
EUR 196.8

CF568 goat anti-rabbit IgG(H+L), 2 mg/mL

20102-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF568 rabbit anti-goat IgG(H+L), 2 mg/mL

20107-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF594 goat anti-rabbit IgG(H+L), 2 mg/mL

20112-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF594 rabbit anti-goat IgG(H+L), 2 mg/mL

20117-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF633 goat anti-rabbit IgG(H+L), 2 mg/mL

20122-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF633 rabbit anti-goat IgG(H+L), 2 mg/mL

20128-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF350 goat anti-rabbit IgG(H+L), 2 mg/mL

20141-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF405M goat anti-rabbit IgG(H+L), 2 mg/mL

20181-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

Biotin goat anti-rabbit IgG(H+L), 2 mg/mL

20185-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF640R goat anti-rabbit IgG(H+L), 2 mg/mL

20202-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF488A chicken anti-rabbit IgG(H+L), 2 mg/mL

20209-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF594 chicken anti-rabbit IgG(H+L), 2 mg/mL

20223-1 50uL
EUR 150
Description: Minimum order quantity: 1 unit of 50uL

CF633 chicken anti-rabbit IgG(H+L), 2 mg/mL

20224-1 50uL
EUR 150
Description: Minimum order quantity: 1 unit of 50uL

CF543 goat anti-rabbit IgG(H+L), 2 mg/mL

20309-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF543 rabbit anti-goat IgG(H+L), 2 mg/mL

20315-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF488A goat anti-rabbit IgG(H+L), 2 mg/mL

20012-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF488A rabbit anti-goat IgG(H+L), 2 mg/mL

20021-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF555 rabbit anti-goat IgG(H+L), 2 mg/mL

20031-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF647 rabbit anti-goat IgG(H+L), 2 mg/mL

20049-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF660C goat anti-rabbit IgG(H+L), 2 mg/mL

20053-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF660R goat anti-rabbit IgG(H+L), 2 mg/mL

20055-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

CF680 rabbit anti-goat IgG(H+L), 2 mg/mL

20068-1 50uL
EUR 158.4
Description: Minimum order quantity: 1 unit of 50uL

CF750 goat anti-rabbit IgG(H+L), 2 mg/mL

20073-1 50uL
EUR 158.4
Description: Minimum order quantity: 1 unit of 50uL

CF405S goat anti-rabbit IgG(H+L), 2 mg/mL

20082-1 50ul
EUR 144
Description: Minimum order quantity: 1 unit of 50ul

CF640R rabbit anti-goat IgG(H+L), 2 mg/mL

20090-1 50uL
EUR 144
Description: Minimum order quantity: 1 unit of 50uL

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1003-1 1ml
EUR 157.2

Biotin Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1005-1 1ml
EUR 157.2

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1006-1 1ml
EUR 157.2

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1054-1 1ml
EUR 279.6

HRP Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1058-1 1ml
EUR 279.6

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1060-1 1ml
EUR 279.6

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1090-1 1ml
EUR 400.8

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1091-1 1ml
EUR 400.8

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1105-1 1mL
EUR 242.4

FITC Conjugated Rabbit Anti-rat IgG (H+L) secondary antibody

BA1108-1 1mL
EUR 279.6

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1110-1 1mL
EUR 242.4

DyLight®488 Conjugated Goat Anti-rabbit IgG (H+L)

BA1127-1 1ml
EUR 400.8

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)

BA1142-1 1ml
EUR 400.8

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody

BM2004-1 1ml
EUR 412.8

Rabbit anti Human IgG (FITC)

43C-CB0944 1 mg
EUR 373.2
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0945 1 mg
EUR 417.6
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0947 1 mg
EUR 408
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0948 1 mg
EUR 500.4
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit anti Human IgG (FITC)

43C-CB0951 2 mg
EUR 327.6
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0952 2 mg
EUR 333.6
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0954 2 mg
EUR 408
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0955 2 mg
EUR 433.2
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit anti Human IgG (FITC)

43C-CB0965 2 mg
EUR 349.2
Description: Rabbit anti Human IgG secondary antibody (FITC)

Rabbit anti Human IgG (rhodamine)

43C-CB0966 2 mg
EUR 349.2
Description: Rabbit anti Human IgG secondary antibody (Rhodamine)

Rabbit anti Human IgG (biotin)

43C-CB0968 2 mg
EUR 430.8
Description: Rabbit anti Human IgG secondary antibody (biotin)

Rabbit anti Human IgG (HRP)

43C-CB0969 2 mg
EUR 442.8
Description: Rabbit anti Human IgG secondary antibody (HRP)

Rabbit Anti-Human IgG FC

C020221-1mg 1mg
EUR 322.8

Rabbit Anti-Human IgG FC

C020221-50mg 50mg
EUR 7218

Rabbit Anti Human IgG-Biotin

E61I00102 1mg
EUR 598.8

Rabbit Anti Human IgG-HRP

E61I00103 1mg
EUR 598.8

Rabbit Anti Human IgG-FITC

E61I00104 1mg
EUR 733.2

Rabbit anti-Saccharomyces Cerevisiae Host Cell Proteins (HCPs) IgG (Low molecular weight fraction, affinity pure)

760-160-11A 100 ug
EUR 548.4

Rabbit anti-Saccharomyces Cerevisiae Host Cell Proteins (HCPs) IgG (High molecular weight fraction, affinity pure)

760-160-12A 100 ug
EUR 548.4

CF488A Rabbit anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL

20071-1 50uL
EUR 164.4
Description: Minimum order quantity: 1 unit of 50uL

CF594 Rabbit anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL

20072-1 50uL
EUR 164.4
Description: Minimum order quantity: 1 unit of 50uL

PAI-1 Antibody

5579-100
EUR 405.6

PAI-1 Peptide

45-142P 0.1 mg
EUR 405.6
Description: (IN) PAI-1 / SERPINE1 Peptide

PAI 1 antibody

20R-1823 100 ug
EUR 807.6
Description: Rabbit polyclonal PAI 1 antibody

PAI 1 Antibody

ABF5176 100 ug
EUR 525.6
These findings counsel that the methods used to induce multi-antigen-targeted mucosal immunity, comparable to IN/IM supply of rAdv-AI, could also be a promising strategy for creating broad protecting vaccines that could be more practical towards the brand new HPAI pandemic strains.

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