We conducted field and pot experiments to investigate the effects of brassinolide on 1-year-old Robinia pseudoacacia L. seedlings. In the field experiment, the roots of the seedlings were soaked in brassinolide solutions containing 0–0.4 mg / l of pure brassinolide before planting. Seedling survival and growth were determined 8 months later. The results showed that soaking the roots in brassinolide before planting significantly increased seedling survival and growth.

The best results were obtained in the treatment with brassinolide of 0.2 mg / l. In the pot experiment, the roots were soaked in 0-0.4 mg / L brassinolide before planting, followed by a foliar application of brassinolide when the seedlings emerged. After the seedlings were established, the soil water content in the pots was regulated to simulate drought conditions and various physiological parameters were measured. The results showed that the treatment with 0.2 mg / l of brassinolide decreased the transpiration rate, the stomatal conductance, and the malondialdehyde content (MDA) of the seedlings growing under moderate or severe water stress compared to the untreated seedlings.

Leaf water content, predawn water potential, soluble sugar content, free proline content, and superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were higher in seedlings. with water stress in the treatment with brassinolide of 0.2 mg / l compared to the control. The results indicate that the application of brassinolide can improve the effects of water stress and improve the drought resistance of Robinia seedlings. Treatment of seedlings with brassinolide can be a useful management tool for afforestation projects in arid and semi-arid areas.

How does brassinolide work in plants?

Brassinolide steroids only affect the plants in which they are found. They do not leave residues that can run down to the water table and do not harm or kill the insects, animals, or humans that consume the plants. We’ve all seen many sci-fi movies in which some plant hormones or fertilizers create powerful mutant plants or insects, but brassinolide hormones simply tell a plant how big to grow and how much seed or fruit to produce, while increasing. the capacity of the plant. immunity and resistance. They are administered to plants in natural doses naturally.

Today, brassinolide steroids are mainly used in agricultural fields that grow cereals. They are available to consumers in liquid or powder form. Brassinolide plant hormones can be used to inoculate seeds before germination to speed up the process. They can also be watered on the roots of plants or used as a foliar feed.

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